Abdelbasset Kaddour1,3,
Djilani Ghemam Amara1,2 
,
Younes Moussaoui3,4,
Ahmed Elkhalifa Chemsa2,5,
Zaid Alia1,2,
Abasse Kamarchou6
For correspondence:- Djilani Amara Email: ghemamamaradjilani@gmail.com Tel:+213658943163
Accepted: 31 August 2022 Published: 30 September 2022
Citation: Kaddour A, Amara DG, Moussaoui Y, Chemsa AE, Alia Z, Kamarchou A. Total phenolic and flavonoid contents of Mentha spicata leaves aqueous extracts in different regions of Algeria and their antioxidant, and antidiabetic activities. Trop J Pharm Res 2022; 21(9):1907-1913 doi: 10.4314/tjpr.v21i9.14
© 2022 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the total phenolic and flavonoid contents of Mentha spicata (MS) leaves aqueous extracts obtained from different regions (El-Oued, Tebessa and El-Tarf) of Algeria, as well as their in vitro antioxidant and antidiabetic activities.
Methods: Aqueous extracts were obtained from the air-dried leaves of MS by maceration followed by filtration and evaporation using a rotary evaporator. Folin-Ciocalteu and Aluminium chloride colorimetric techniques were used to determine the total phenolic and flavonoid contents, respectively. The radical DPPH, ABTS, and FRAP tests were used to determine their antioxidant activities, while the in vitro inhibition percentages of α-amylase and α-glucosidase enzymes were used to assess the anti-diabetic activity.
Results: The El-Oued's extract exhibited the highest total phenolic content (108.94 mg GAE/g dry extract (DE)), while the highest total flavonoid content (0.039 mg QE/g DE) was found in El-Tarf's extract. The radical DPPH and FRAP scavenging activity of the El-Oued extract exhibited the highest inhibition activities (IC50 = 102.5 and 289.5 µg/mL), respectively, while the ABTS inhibition activity of the El-Tarf extract exhibited a maximum IC50 value of 111 ± 2.8 µg/mL. The MS extract of the El-Oued region had the highest α-amylase and α-glucosidase enzyme activities with IC50 values of 121.4 and 216.9 µg/mL, respectively.
Conclusion: The leaves of Mentha spicata exhibit high phenolic and flavonoid contents, along with significant antioxidant and antidiabetic properties. This study reveals that Mentha spicata flavonoid and phenolic contents as well as other properties vary by region.
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